Download (zip file contains SapTrap Builder App + PDF user guide):

Mac Windows Non-App Version

OSX 10.12.6 and later Older OSX, etc*

*If the application bundles don’t work, you can install python 3.6 and run the scripts directly.

Download the ‘Non-App Version’ folder and follow the ‘Setup Instructions’ located in the folder.

Instructional Videos (youtube):

General Program Operation

Making Large Deletions

Making Point Mutations

Program Features:

- Design reagents for CRISPR tags, deletions and mutations in minutes

- Enter a genomic sequence and let the program guide you through reagent design

- Intuitive sequence display illustrates all components of your edit

- Simplifies addition of necessary silent mutations as well as coding changes

- Catches common design errors

- Outputs ready-to-order sequences

- Built for SapTrap, yet compatible with any DNA assembly protocol

- Works on PC and Mac

Questions/Comments/Trouble

email me at matt dot schwartz at utah dot edu