T3 ATTAACCCTCACTAAAGGGA primer_bind cyan green 0 M13-fwd TGTAAAACGACGGCCAGT primer_bind cyan green 0 M13-rev CAGGAAACAGCTATGACCATG primer_bind cyan green 0 T7 gtgaattgACTTATATGCGTCTATTTATGTAGGATGAAAGGTAGTCTAGTACCTCCTGTGATATTATCCCATTCCATGCGGGGTATCGTATGCTTCCTTCAGCACTACCCTTTAGCTGTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCTATCATTTCCTTTGATATTGGATCA rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCSGGCTTTCCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGCCCCATTAAGCGCGGCGGGTGTGGTGGTTACGCCCAGCGTGACCCCTACACTTCCCACCGCCCTAGCCCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATGGGGGCATCCGTTTACCCTTCCGATTTACTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACCCTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTGGAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGG>GCTATTCGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCSGGCTTTCCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCTTTCCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCSGGCTTTCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCSGGCTTTCCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAAGAGTCCACTATTAAAGAACGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 M13 origin 3/28/23 ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCYTAGCGCCCGCTCCTTTCGCTTTCTTCCCTYCCTTTCTCGCCACGTTCGCSGGCTTTCCCCGTCAAGCTCTAAATCGGGGSMTCCCTTTAGGGTTCCGATTTAGWGCTTTACGGCACCTCGACCSCAAAAAACTTGATTWGGGTGATGGTTCACGTAGTGGGCGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTKGAACAACACTCAACYCTATCKCGGKCTATTCTTTTGATTTATAAGGGATKTTGSSGATTTCGG>YCTATTGGTTAAAAAATGAGCTGATTTAACAAAWATTTAACGCGAATTTTAACAAAMTAKTAACGYTTACAATTT rep_origin gray50 gray50 0 R6Kgamma origin 3/28/23 GTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCYTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTSAGTGACACAGGAACACTTAACGGCTGACA rep_origin gray50 gray50 0 oriV 3/28/23 CTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAGGGGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGGTGAGCGTCGSAAAGGCGC rep_origin gray50 gray50 0 oriV 3/28/23 CTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAGGGGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGGTGAGCGTCGSAAAGGCGC rep_origin gray50 gray50 0 oriV 3/28/23 CTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAGGGGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGGTGAGCGTCGSAAAGGCGC rep_origin gray50 gray50 0 pBBR1(oriV) 3/28/23 GCGGCCACCGGCTGGCTCGCTTCGCTCGGCCCGTGGACAACCCTGCTGGACAAGCTGATGGACAGGCTGCGCCTGCCCACGAGCTTGACCACAGGGATTGCCCACCGGCTACCCAGCCTTCGACCACATACCCACCGGCTCCAACTGCGCGGCCTGCGGCCTTGCCCCATCAATTTTTTTAATTTTCTCTGGGGAAAAGCCTCCGGCCTGCGGCCTGCGCGCTTCGCTTGCCGGTTGGACACCAAGTGGAAGGCGGGTCAAGGCTCGCGCAGCGACCGCGCAGCGGCTTGGCCTTGACGCGCCTGGAACGACCCAAGCCTATGCGAGTGGGGGCAGTCGAARGGCGAAGCCCGCCCGCCTGCCCCCCGAGCCTCACGGCGGCGAGTGCGGGGGTTCCAAGGGGGCAGCGCCACCTTGGGCAAGGCCGAAGGCCGCGCAGTCGATCAACAAGCCCCGGAGGGGCCACTTTTTGCCGGAGGGGGAGCCGCGCCGAAGGCGTGGGGGAACCCCGCAGGGGTGCCCTTCTTTGGGCACCAAAGAACTAGATATAGGGCGAAATGCGAAAGACTTAAAAATCAACAACTTAAAAAAGGGGGGTACGCAACAGCTCATTGCGGCACCCCCCGCAATAGCTCATTGCGTAGGTTAAAGAAAATCTGTAATTGACTGCCACTTTTACGCAACGCATAATTGTTGTCGCGCTGCCGAAAAGTTGCAGCTGATTGCGCATGGTGCCGCAACCGTGCGGCACCCCTACCGCATGGAGATAAGC rep_origin gray50 gray50 0 pBBR1(oriV) 3/28/23 GCGGCCACCGGCTGGCTCGCTTCGCTCGGCCCGTGGACAACCCTGCTGGACAAGCTGATGGACAGGCTGCGCCTGCCCACGAGCTTGACCACAGGGATTGCCCACCGGCTACCCAGCCTTCGACCACATACCCACCGGCTCCAACTGCGCGGCCTGCGGCCTTGCCCCATCAATTTTTTTAATTTTCTCTGGGGAAAAGCCTCCGGCCTGCGGCCTGCGCGCTTCGCTTGCCGGTTGGACACCAAGTGGAAGGCGGGTCAAGGCTCGCGCAGCGACCGCGCAGCGGCTTGGCCTTGACGCGCCTGGAACGACCCAAGCCTATGCGAGTGGGGGCAGTCGAARGCGAAGCCCGCCCGCCTGCCCCCCGAGCCTCACGGCGGCGAGTGCGGGGGTTCCAAGGGGGCAGCGCCACCTTGGGCAAGGCCGAAGGCCGCGCAGTCGATCAACAAGCCCCGGAGGGGCCACTTTTTGCCGGAGGGGGAGCCGCGCCGAAGGCGTGGGGGAACCCCGCAGGGGTGCCCTTCTTTGGGCACCAAAGAACTAGATATAGGGCGAAATGCGAAAGACTTAAAAATCAACAACTTAAAAAAGGGGGGTACGCAACAGCTCATTGCGGCACCCCCCGCAATAGCTCATTGCGTAGGTTAAAGAAAATCTGTAATTGACTGCCACTTTTACGCAACGCATAATTGTTGTCGCGCTGCCGAAAAGTTGCAGCTGATTGCGCATGGTGCCGCAACCGTGCGGCACCCTACCGCATGGAGATAAGC rep_origin gray50 gray50 0 incP origin of transfer 3/28/23 CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCC oriT gray50 gray50 0 oriS 3/28/23 CTTCTTCTGAGGGCAATTTGTCACAGGGTTAAGGGCAATTTGTCACAGACAGGACTGTCATTTGAGGGTGATTTGTCACACTGAAAGGGCAATTTGTCACAACACCTTCTCTAGAACCAGCATGGATAAAGGCCTACAAGGCGCTCTAAAAAAGAAGATCTAAAAACTATAAAAAAAATAATTATAAAAATATCCCCGTGGATAAGTGGATAACCCCAAG rep_origin gray50 gray50 0 pSC101 origin 3/28/23 GGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTC rep_origin gray50 gray50 0 TrfA 3/28/23 MNRTFDRKAYRQELIDAGFSAEDAETIA[NS]RTVMRAPRETFQSVGS[IM]VQQATAKIERDSVQLA[SP]P[TA]LPAPSAAVERSRRLEQEA[AT]GLAKSMTIDTRGTMTTKKRKTAGE[GD]LAKQVSE[VA]KQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTRNKKIPREAL[KQ]NKVIFHVNKDVKITYTGVELRADDDELVW[LQ]QVLEYAKRTPIGEPITFTFYELCQDLGWSINGRYYTKAEECLSRLQATAM[GD]FTSDRVGHLESVSLLHRFRVLDRGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVN[ED]DLVHCKR CDS gray50 gray50 1 SacB 3/28/23 MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKY[KQ]VPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK CDS gray50 gray50 1 SacB promoter 4/14/23 CACATATACg promoter #346ee0 #346ee0 0 T7 promoter 4/14/23 taatacgactcactatag>g promoter #346ee0 #346ee0 0 CAP binding site 4/14/23 ATGAGTGAGCTAACTCACATTA misc_feature #6495ed #6495ed 0 cos Site 4/14/23 TTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCGTCAAATAATCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACTTAACGTAAAARCAACTTCAGACAATACAAATCAGCGACACTGAATACGGGGCAACCTCATGTC misc_binding #6495ed #6495ed 0 cos Site 4/14/23 TTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCGTCAAATAATCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACTTAACGTAAAARCAACTTCAGACAATACAAATCAGCGACACTGAATACGGGGCAACCTCATGTC misc_binding #6495ed #6495ed 0 lambda t0 terminator 4/14/23 ATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTC regulatory:terminator #b00f3d #b00f3d 0 lambda tL3 terminator 4/14/23 CTACTGGTATTGGCACAAACCTGATTCCAATTTGAGCAAGGCTATGTGCCATCTCGATACTCGTTCTTAACTCAACAGAAGATGCTTTGTGCATACAGCCCCTCGTTTATTATTTATCTCCTCAGCCAGCCGCTGTGCTTTCAGTGGATTTCGGATAACAGAAAGGCCGGGAAATACCCAGCCTCGCTTTGTAACGGAGTAGAGACGAAAGTGATTGCGCCTACCCGGATATTATCGTGAGGATGCG regulatory:terminator #b00f3d #b00f3d 0 lambda tL3 terminator 4/14/23 CTACTGGTATTGGCACAAACCTGATTCCAATTTGAGCAAGGCTATGTGCCATCTCGATACTCGTTCTTAACTCAACAGAAGATGCTTTGTGCATACAGCCCCTCGTTTATTATTTATCTCCTCAGCCAGCCGCTGTGCTTTCAGTGGATTTCGGATAACAGAAAGGCCGGGAAATACCCAGCCTCGCTTTGTAACGGAGTAGACGAAAGTGATTGCGCCTACCCGGATATTATCGTGAGGATGCG regulatory:terminator #b00f3d #b00f3d 0 rrnB T1 terminator 4/14/23 TGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT regulatory:terminator #b00f3d #b00f3d 0 rrnB T2 terminator 4/14/23 AGAAGGCCATCCTGACGGATGGCCTTTT regulatory:terminator #b00f3d #b00f3d 0 T7 terminator 4/14/23 CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG regulatory:terminator #b00f3d #b00f3d 0 T7Te terminator 4/14/23 GGCTCACCTTCGGGTGGGCCTTT>CTGCG regulatory:terminator #b00f3d #b00f3d 0 tonB terminator 4/14/23 AGTCAAARRCCTCCGRYCGGAGGCTTTTGACT regulatory:terminator #b00f3d #b00f3d 0 bom 4/14/23 CCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAMTGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCG misc_feature #41FF00 #41FF00 0 bom 4/14/23 CCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATMTGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCG misc_feature #41FF00 #41FF00 0 bom 4/14/23 CCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATMTGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCG misc_feature #41FF00 #41FF00 0 pelB signal sequence 4/14/23 MKYLLPTAAAGLLLLAAQPAMA sig_peptide #41FF00 #41FF00 1 LacZ-alpha MTMIT&XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CDS #41FF00 #41FF00 1 NeoR/KanR MGS[MAG][LMIA]A CDS #41FF00 #41FF00 1 CAT/CamR MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPE[LF]RMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLEDPAY CDS #41FF00 #41FF00 1 CAT/CamR MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPE[LF]RMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQFLCMRPIRKPPLPARWPIH CDS #41FF00 #41FF00 1 ZeoR/BleoR M[SA]K[IL]TSAVPVLTARDVAGAVEFWTDRLGFSRDFVE[ND]DFAGVVRDDVTLFISAVQDQVVPDNTLAWV[LW]VRGLDELYAEWSEVVSTNFRDASGP[SA]MTEIGEQPWG[KQR]EFALR[ED]PAGNCVHFVAEEQD CDS #41FF00 #41FF00 1 ZeoR/BleoR M[SA]K[IL]TSAVPVLTARDVAGAVEFWTDRLGFSRDFVE[ND]DFAGVVRDDVTLFISAVQDQDQVVPDNTLAWV[LW]VRGLDELYAEWSEVVSTNFRDASGP[SA]MTEIGEQPWG[KQR]EFALR[ED]PAGNCVHFVAEEQD CDS #41FF00 #41FF00 1 ccdB MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV[PS]RELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI CDS #41FF00 #41FF00 1 Tet(B) MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGVLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLEFIADSSAFAFLAFISEGWLDFPVLILLAGGGIALPALQGVMSIQTKSHEQGALQGLLVSLTNATGVIGPLLFTVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQETSA CDS #41FF00 #41FF00 1 Tet(A) MSTNLSVIKNPRVQSDQRRLVRRPDV[DR][AR][KE][EKM][GV][EG][AR][NV] CDS #41FF00 #41FF00 1 GentamycinR M[LV]RSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLP[RK]FEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT CDS #41FF00 #41FF00 1 Bleomycin/ZeocinR M[MD][DR][AR][KE][EKM][GV][EG][AR][NV] CDS #41FF00 #41FF00 1 Renilla-firefly Luciferase MAGMDELYK CDS #00FF00 #00FF00 1 GFP (S65T) MGMDELYK CDS #00FF00 #00FF00 1 EGFP MVGMDELYK CDS #00FF26 #00FF26 1 mCherry MVGMDELYK CDS #FF1100 #FF1100 1 firefly Luciferase [ET][IS][AK][VL] CDS #41FF00 #41FF00 1 Metridia longa Luciferase MDIKVVFTLVFSALVQAKSTEFDPNIDIVGLEGKFGITNLETDLFTIWETMEVMIKADIADTDRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTRGCLICLSKIKCTAKMKVYIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQFIAQVDRCASCTTGCLKGLANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR CDS #41FF00 #41FF00 1 Nanoluc MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA CDS #41FF00 #41FF00 1 ErythromycinR M-1 CDS #41FF00 #41FF00 1 8xHis -1<[ACDEFGIKLMNPQRSTVWY]HHHHHHHH[ACDEFGIKLMNPQRSTVWY*]>-1 CDS #41FF00 #41FF00 1 10xHis -1<[ACDEFGIKLMNPQRSTVWY]HHHHHHHHHH[ACDEFGIKLMNPQRSTVWY*]>-1 CDS #41FF00 #41FF00 1 Myc tag EQKLISEEDL CDS #41FF00 #41FF00 1 HA tag -1<[CDEFGHIKLMNPQRSTVWY]YPYDVPDYA CDS #41FF00 #41FF00 1 3xHA tag YPYDVPDYAYPYDVPDYAYPYDVPDYA CDS #41FF00 #41FF00 1 HSV tag QPELAPEDPED CDS #41FF00 #41FF00 1 FLAG tag -1<[ACDEFGHILMNPQRSTVWY]DYKDDDDK CDS #41FF00 #41FF00 1 3xFLAG tag DYKDDDDKDYKDDDDKDYKDDDDK CDS #41FF00 #41FF00 1 STREP tag WSHPQFEK CDS #41FF00 #41FF00 1 twinSTREP tag SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK CDS #41FF00 #41FF00 1 MISTIC tag MFCTFFEKHHRKWDILLEKSTGVMEAMKVTSEEKEQLSTAIDRMNEGLDAFIQLYNESEIDEPLIQLDDDTAELMKQARDMYGQEKLNEKLNTIIKQILSISVSEEGEKE CDS #41FF00 #41FF00 1 SIII tag WSHPQFEKGGGSGGGSGGGSWSHPQFEK CDS #41FF00 #41FF00 1 STREP tag AWAHPQPGG CDS #41FF00 #41FF00 1 AU1 tag DTYRYI CDS #41FF00 #41FF00 1 AU5 tag TDFYLK CDS #41FF00 #41FF00 1 Glu-Glu tag EYMPME CDS #41FF00 #41FF00 1 OLLAS tag SGFANELGPRLMGK CDS #41FF00 #41FF00 1 T7 tag MASMTGGQQMG CDS #41FF00 #41FF00 1 V5 tag GKPIPNPLLGLDST CDS #41FF00 #41FF00 1 VSV-G tag YTDIEMNRLGK CDS #41FF00 #41FF00 1 C tag EPEA CDS #41FF00 #41FF00 1 E tag GAPVPYPDPLEPR CDS #41FF00 #41FF00 1 S tag KETAAAKFERQHMDS CDS #41FF00 #41FF00 1 Avi tag GLNDIFEAQKIEWHE CDS #41FF00 #41FF00 1 HSV tag SQPELAPEDPED CDS #41FF00 #41FF00 1 KT3 tag KPPTPPPEPET CDS #41FF00 #41FF00 1 TK15 tag [RK]TV[IL]HGESNSA[IL][IL][IL]GPR CDS #41FF00 #41FF00 1 ALFA tag SRLEEELRRRLTE CDS #41FF00 #41FF00 1 iCAP tag MIKIATRKYLGKQNVYGIGVERDHNFALKNGFIAHN CDS #41FF00 #41FF00 1 NE tag TKENPRSNQEESYDDNES CDS #41FF00 #41FF00 1 Rho1D4 tag TETSQVAPA CDS #41FF00 #41FF00 1 Softag 1 SLAELLNAGLGGS CDS #41FF00 #41FF00 1 Softag 3 TQDPSRVG CDS #41FF00 #41FF00 1 Spot tag PDRVRAVSHWSS CDS #41FF00 #41FF00 1 tetracysteine tag CCPGCC CDS #41FF00 #41FF00 1 TY tag EVHTNQDPLD CDS #41FF00 #41FF00 1 Isopeptag TDKDMTITFTNKKDAE CDS #41FF00 #41FF00 1 SpyTag AHIVMVDAYKPTK CDS #41FF00 #41FF00 1 SnoopTag KLGDIEFIKVNK CDS #41FF00 #41FF00 1 DogTag DIPATYEFTDGKHYITNEPIPPK CDS #41FF00 #41FF00 1 SdyTag DPIVMIDNDKPIT CDS #41FF00 #41FF00 1 TEV cleavage site ENLYFQ[GS] CDS #41FF00 #41FF00 1 thrombin cleavage site LVPRGS CDS #41FF00 #41FF00 1 Factor Xa cleavage site**too short?** I[ED]GR CDS #41FF00 #41FF00 1 enterokinase cleavage site -1<[ACDEFGHIKLMNPQRSTVW]DDDD[KR] CDS #41FF00 #41FF00 1 Xpress cleavage site DLYDDDDK CDS #41FF00 #41FF00 1 TVMV cleavage site TVRFQS CDS #41FF00 #41FF00 1 HRV 3C cleavage site LEVLFQGP CDS #41FF00 #41FF00 1 BCCP MASSSFSVTSPAAAASVYAVTQTSSHFPIQNRSRRVSFRLSAKPKLRFLSKPSRSSYPVVKAQSNKVSTGASSNAAKVDGPSSAEGKEKNSLKESSASSPELATEESISEFLTQVTTLVKLVDSRDIVELQLKQLDCELVIRKKEALPQPQAPASYVMMQQPNQPSYAQQMAPPAAPAAAAPAPSTPASLPPPSPPTPAKSSLPTVKSPMAGTFYRSPAPGEPPFIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPLFVVQP CDS #41FF00 #41FF00 1 Chitin-binding domain TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ CDS #41FF00 #41FF00 1 Calmodulin-binding peptide RWKKNFIAVSAANRFKKIS CDS #41FF00 #41FF00 1 Calmodulin-binding peptide GVMPREETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLA CDS #41FF00 #41FF00 1 streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP CDS #41FF00 #41FF00 1 T2A GSGEGRGSLLTCGDVEENPGP CDS #41FF00 #41FF00 1 P2A GSGATNFSLLKQAGDVEENPGP CDS #41FF00 #41FF00 1 E2A GSGQCTNYALLKLAGDVESNPGP CDS #41FF00 #41FF00 1 F2A GSGVKQTLNFDLLKLAGDVESNPGP CDS #41FF00 #41FF00 1 maltose-binding protein (MPB) M[EK]IKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEEL[AV]KDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT CDS #41FF00 #41FF00 1 glutathione S-transferase MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK CDS #41FF00 #41FF00 1 Thioredoxin MGSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGS CDS #41FF00 #41FF00 1 CMV promoter CGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTNCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTANGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACANCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAANAACNCCGCCCCNTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGA promoter cornflower blue cornflower blue 0 SV40 early promoter TGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAA promoter cornflower blue cornflower blue 0 hGH polyA signal CGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTG polyA_signal pink pink 0 attB5 CMASTTTGTATACAAAAGYWG misc_recomb #dda0dd #dda0dd 0 attB4 CMASTTTGTATAGAAAAGYWG misc_recomb #dda0dd #dda0dd 0 attB3 CMASTWTGTATAATAAAGYWG misc_recomb #dda0dd #dda0dd 0 AttB2 CMASTTTGTACAAGAAAGYWG misc_feature #dda0dd #dda0dd 0 AttB1 CMASTTTGTACAAAAAAGYWG misc_feature #dda0dd #dda0dd 0 attP5 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTATACAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 attP4 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTATAGAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 attP3 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTWTGTATAATAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 AttP2 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTACAAGAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb plum plum 0 AttP1 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTACAAAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb plum plum 0 attL5 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTATACAAAAGYWG misc_recomb #dda0dd #dda0dd 0 attL4 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTATAGAAAAGYWG misc_recomb #dda0dd #dda0dd 0 attL3 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTWTGTATAATAAAGYWG misc_recomb #dda0dd #dda0dd 0 AttL2 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTACAAGAAAGYWG misc_recomb plum plum 0 AttL1 AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAMATTGATRAGCAATGCTTTYTTATAATGCCMASTTTGTACAAAAAAGYWG misc_recomb plum plum 0 attR5 CMASTTTGTATACAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 attR4 CMASTTTGTATAGAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 attR3 CMASTWTGTATAATAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb #dda0dd #dda0dd 0 AttR2 CMASTTTGTACAAGAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb plum plum 0 AttR1 CMASTTTGTACAAAAAAGYWGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATSCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTA misc_recomb plum plum 0 ccdB ATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCNCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATA CDS yellow yellow 0